PNPP NEW
| Price | $37 |
| Package | 1g |
| Min. Order: | |
| Supply Ability: | 10g |
| Update Time: | 2025-07-08 |
Product Details
| Product Name: PNPP | CAS No.: 4264-83-9 |
| Purity: 99.83% | Supply Ability: 10g |
| Release date: 2025/07/08 |
Product Introduction
Bioactivity
| Name | PNPP |
| Description | PNPP is a non-proteinaceous chromogenic substrate for alkaline and acid phosphatases used in ELISA and conventional spectrophotometric assays. |
| In vitro | Instructions I. Solution preparation 1. Stock solution preparation: Dissolve PNPP powder in an appropriate buffer, usually at a concentration of 5–10 mM. It is recommended to use a buffer containing 50 mM Tris-HCl and 10 mM MgCl?, with the pH adjusted to 9.5 to suit the activity of alkaline phosphatase. 2. Working solution preparation: Dilute the stock solution to the required concentration, usually 2–5 mM, according to experimental needs. II. Operation steps 1. Reaction conditions: Temperature: 37°C. Time: 10–30 minutes, the specific time needs to be optimized according to the experiment. 2. Detection method: 1) Under the action of the enzyme, PNPP is hydrolyzed to produce yellow p-nitrophenol, which has a maximum absorption peak at 405 nm. 2) Use a spectrophotometer to measure the absorbance at 405 nm to evaluate the activity of the enzyme. Note: Fresh preparation: PNPP solution should be prepared before use and avoid long-term storage to prevent hydrolysis. Store away from light: PNPP is light-sensitive, so strong light exposure should be avoided during operation. Termination of reaction: In ELISA experiments, an equal volume of 0.1 M NaOH or 0.1 M EDTA can be added after the reaction is completed to terminate the reaction. |
| Storage | store at low temperature,keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. |
| Solubility Information | H2O : 160 mg/mL (608.25 mM), Sonication is recommended. |
| Keywords | PNPP |
Company Profile Introduction
Target Molecule Corp. (TargetMol) is a global high-tech enterprise, headquartered in Boston, MA, specializing in chemical and biological research product and service to meet the research needs of global customers.
TargetMol has evolved into one of the biggest global compound library and small molecule suppliers and a customer based on 40+ countries. TargetMol offers over 80 types of compound libraries and a wide range of high-quality research chemicals including inhibitors, activator, natural compounds, peptides, inhibitory antibodies, and novel life-science kits, for laboratory and scientific use. Besides, virtual screening service is also available for customers who would like to conduct the computer-aided drug discovery.
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United States- Since: 2011-01-07
- Address: 36 Washington Street, Wellesley Hill, MA
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