Neutral red NEW
| Price | $41 |
| Package | 500mg |
| Min. Order: | |
| Supply Ability: | 10g |
| Update Time: | 2025-07-28 |
Product Details
| Product Name: Neutral red | CAS No.: 553-24-2 |
| Purity: 99.15% | Supply Ability: 10g |
| Release date: 2025/07/28 |
Product Introduction
Bioactivity
| Name | Neutral red |
| Description | Neutral red is a vital dye, is used as an indicator and biological stain. |
| Cell Research | Cell Neutral red uptake assay Procedure: 1.Cells were cultured in a 96-well plate under normal conditions until they reached a confluency of 70-80%. 2.The neutral red working solution (prepared the day before the neutral red uptake assay and incubated overnight in a 37°C cell culture incubator) was centrifuged at 4,000 g for 5 minutes at room temperature (20-30°C) to remove any precipitate. 3.200 μL PBS was added to each well of the 96-well plate to rinse the cells. 4.100 μL of neutral red working solution was added to each well using a dispenser. 5.Incubated in a 37°C incubator (5% CO2) for 2-4 hours. Note: For RD and MRC-5 cell lines, the optimal incubation time is 2 hours, and longer incubation times will result in significant sensitivity loss. For Vero E6 and A549-hACE2 cell lines, the incubation time can be up to 4 hours without affecting the detection sensitivity; 6. Add 200 μL PBS to each well to wash the cells and remove the neutral red working solution. 7. Aspirate the wash buffer and gently pour the remaining buffer on a paper towel or let it air dry. Note: The wells need to be completely dry before adding neutral red destaining solution. 8. Add 100 μL of neutral red destaining solution (usually 50% ethanol, 1% acetic acid or DMSO) to each well. Shake the plate quickly at 200 rpm on a microplate shaker for 15 minutes to allow the destaining solution to completely extract neutral red from the cells and form a uniform solution. 9. Measure the absorbance of the neutral red extract at 540 nm using a spectrophotometer. |
| Storage | keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature. |
| Solubility Information | DMSO : 49 mg/mL (169.68 mM), Sonication is recommended. |
| Keywords | Neutral red | Inhibitor | inhibit |
Company Profile Introduction
Target Molecule Corp. (TargetMol) is a global high-tech enterprise, headquartered in Boston, MA, specializing in chemical and biological research product and service to meet the research needs of global customers.
TargetMol has evolved into one of the biggest global compound library and small molecule suppliers and a customer based on 40+ countries. TargetMol offers over 80 types of compound libraries and a wide range of high-quality research chemicals including inhibitors, activator, natural compounds, peptides, inhibitory antibodies, and novel life-science kits, for laboratory and scientific use. Besides, virtual screening service is also available for customers who would like to conduct the computer-aided drug discovery.
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United States- Since: 2011-01-07
- Address: 36 Washington Street, Wellesley Hill, MA
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