Product Details
| Product Name:
Laurdan |
CAS No.:
74515-25-6 |
| Purity:
99.02% |
Supply Ability:
10g |
| Release date:
2025/07/08 |
Product Introduction
Bioactivity
| Name | Laurdan |
| Description | Laurdan (6-Dodecanoyl-2-dimethylaminonaphthalene) is a fluorescent membrane probe that is spectrally sensitive to the phospholipid phase of the cell membrane to which it binds. |
| Cell Research | Laurdan as a biosensor to monitor changes in membrane water content during NIH3T3 cell migration
I. Solution preparation
1. Preparation of storage solution: Dissolve Laurdan in dimethyl sulfoxide (DMSO) to prepare a 1.8 mM stock solution.
2. Preparation of working solution: Dilute the Laurdan stock solution at a dilution of 1:1000.
II. Operation steps
1. Culture NIH3T3 cells in Dulbecco's modified Eagle medium supplemented with 10% fetal bovine serum, 5 mL Pen-Strep, and 2.5 mL 1 M HEPES.
2. Seed freshly isolated NIH3T3 cells into 35 mm Mattek glass-bottom culture dishes coated with fibronectin.
3. Incubate the cells with Laurdan at 37°C for 40 min.
4. Observe using an LSM710 META laser scanning microscope.
5. Calibration of the system and phasor plot of FLIM data by measuring fluorescein (pH 11). |
| Storage | keep away from direct sunlight,store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. |
| Solubility Information | DMSO : 10 mg/mL (28.29 mM), Sonication is recommended.
|
| Keywords | Laurdan | Inhibitor | inhibit |
| Related Compound Libraries | Human Metabolite Library |
Company Profile Introduction
Target Molecule Corp. (TargetMol) is a global high-tech enterprise, headquartered in Boston, MA, specializing in chemical and biological research product and service to meet the research needs of global customers.
TargetMol has evolved into one of the biggest global compound library and small molecule suppliers and a customer based on 40+ countries. TargetMol offers over 80 types of compound libraries and a wide range of high-quality research chemicals including inhibitors, activator, natural compounds, peptides, inhibitory antibodies, and novel life-science kits, for laboratory and scientific use. Besides, virtual screening service is also available for customers who would like to conduct the computer-aided drug discovery.
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Recommended supplier
| Product name |
Price |
|
Suppliers |
Update time |
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|
$0.00/100mg |
VIP2Y
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Xian confluore Biological Technology Co., Ltd.
|
2024-03-30 |
United States