午夜插插,噜噜噜影院,啪啪伊人网,欧美熟夫,景甜吻戏视频,男人强操性感蕾丝美女视频在线网站,日本美女跳舞视频

Welcome to chemicalbook!
+1 (818) 612-2111
RFQ
Try our best to find the right business for you.
Do not miss inquiry messages Please log in to view all inquiry messages.

Welcome back!

RFQ
skype
MY Account
Top
Postion:Product Catalog >Hydroxyphenyl Fluorescein
Hydroxyphenyl Fluorescein
  • Hydroxyphenyl Fluorescein

Hydroxyphenyl Fluorescein NEW

Price Get Latest Price
Package 1mg 5mg
Min. Order:
Supply Ability: 10g
Update Time: 2025-07-17

Product Details

Product Name: Hydroxyphenyl Fluorescein CAS No.: 359010-69-8
Supply Ability: 10g Release date: 2025/07/17

Product Introduction

Bioactivity

NameHydroxyphenyl Fluorescein
DescriptionHydroxyphenyl Fluorescein (HPF) is a cell-permeable fluorescent dye for the detection of highly reactive oxygen species with little or no intrinsic fluorescence.Hydroxyphenyl Fluorescein can be used in fluorescent enzyme labeling and flow cytometry, and can be used to Neurodegeneration and aging. Fluorescence wavelength: Ex/Em = 490/515 nm.
Cell ResearchOperation steps: a. Preparation of stock solution: Prepare 10 mM HPF with DMSO; (it is recommended to store at -20 ℃ or -80 ℃ in the dark after aliquoting) b. Preparation of HPF working solution Dilute the stock solution and dissolve it in serum-free cell culture medium or PBS to prepare 1-10 μM HPF working solution; (select the appropriate working solution concentration according to experimental requirements and prepare it for immediate use) c. Cell staining 1. Suspended cells (6-well plate) a. Centrifuge at 1000 g for 3-5 minutes at 4℃, and then discard the supernatant. Wash twice with PBS for 5 minutes each. The cell density is 1×106/mL; b. Add 1 mL of working solution and incubate at room temperature for 15 minutes; c. Centrifuge at 4℃ and 400 g for 3-4 minutes, and discard the supernatant; d. Wash twice with PBS, 5 minutes each time; e. Resuspend cells with serum-free cell culture medium or PBS. Observe with fluorescence microscope or flow cytometer. 2. Adherent cells a. Culture adherent cells on sterile coverslips; b. Remove coverslips from culture medium and aspirate excess culture medium; c. Add 100 μL working solution, shake gently to completely cover cells, and incubate at room temperature for 15 minutes; d. Wash twice with culture medium, 5 minutes each time. Observe with fluorescence microscope or flow cytometer.
Storagestore at low temperature,keep away from direct sunlight | store at -20°C | Shipping with blue ice/Shipping at ambient temperature.
Solubility InformationDMSO : 10 mg/mL (23.56 mM), Sonication is recommended.
DMF : 8 mg/mL (18.85 mM), Sonication is recommended.
Ethanol : 8 mg/mL (18.85 mM), Sonication is recommended.

Company Profile Introduction

Target Molecule Corp. (TargetMol) is a global high-tech enterprise, headquartered in Boston, MA, specializing in chemical and biological research product and service to meet the research needs of global customers. TargetMol has evolved into one of the biggest global compound library and small molecule suppliers and a customer based on 40+ countries. TargetMol offers over 80 types of compound libraries and a wide range of high-quality research chemicals including inhibitors, activator, natural compounds, peptides, inhibitory antibodies, and novel life-science kits, for laboratory and scientific use. Besides, virtual screening service is also available for customers who would like to conduct the computer-aided drug discovery.

You may like

Recommended supplier

Product name Price   Suppliers Update time
$79.00/1kg
VIP1Y
Hebei Zhuanglai Chemical Trading Co Ltd
2024-11-22
$56.00/5g
VIP3Y
TargetMol Chemicals Inc.
2025-07-09
$6.00/1kg
VIP1Y
HebeiShuoshengImportandExportco.,Ltd
2024-08-07
$0.00/1mg
VIP2Y
Xian confluore Biological Technology Co., Ltd.
2024-05-10
  • Since: 2011-01-07
  • Address: 36 Washington Street, Wellesley Hill, MA
INQUIRY