Product Information

• Product Code：E062018

• English Name：Eravacycline Difference phase isomer

• English Alias：(4R,4aS,5aR,12aS)-4-(dimethylamino)-7-fluoro-3,10,12,12a-tetrahydroxy-1,11-dioxo-9-(2-(pyrrolidin-1-yl)acetamido)-1,4,4a,5,5a,6,11,12a-octahydrotetracene-2-carboxamide

• CAS No.：1431558-65-4

• Molecular Formula：C??H??FN?O?

• Molecular Weight：558.56

Advantages

• High-Purity Isomer Reference Standard：Confirmed by HPLC (≥99.0%), NMR (with chiral shift reagents), HRMS, and X-ray crystallography for stereoconfiguration, suitable for precise analysis of Eravacycline difference phase isomers.

• Stability Assurance：Stable for 36 months at -20℃ under light-protected, sealed storage; degradation rate <0.2% in methanol-water solution within 6 months.

Applications

• Quality Control Testing：Used for UPLC-MS/MS detection of difference phase isomers in Eravacycline API and formulations, controlling isomer content to meet ICH Q3A standards (≤0.5%).

• Process Optimization Research：Monitors difference isomerization side reactions during Eravacycline synthesis, reducing generation by >60% by adjusting hydrogenation temperature (e.g., 10-15℃) and catalyst dosage (e.g., Pd/C).

• Method Validation：Serves as a standard for developing isomer separation methods, verifying UPLC resolution (≥3.0) and LOD (0.01 ng/mL).

Background Description

Research Status

• Detection Technology：UPLC-MS/MS with chiral C18 column (1.7μm) and 0.1% phosphoric acid-water-acetonitrile gradient elution achieves separation within 4 minutes, with LOD of 0.005 ng/mL for high-sensitivity analysis.

• Formation Mechanism：Formed by epimerization of Eravacycline parent nucleus during hydrogenation; optimizing reaction pressure (e.g., 5-10 atm) and solvent polarity (e.g., ethanol-water system) inhibits side reactions.

• Safety Evaluation：In vitro antibacterial assays show MIC of 4 μg/mL against E. coli (Eravacycline MIC=0.5 μg/mL), with low toxicity but requiring ≤0.5% limit. Accelerated stability testing is ongoing to monitor isomerization rates under different pH conditions