Cyclobenzaprine USP Related Compound A; 18029-54-4

Product Information

• Product Code：C140001

• English Name：Cyclobenzaprine USP Related Compound A

• English Alias：5-(3-(dimethylamino)propyl)-5H-dibenzo[a,d][7]annulen-5-ol

• CAS No.：18029-54-4

• Molecular Formula：C??H??NO

• Molecular Weight：293.40

Advantages

• High-Purity Reference Standard：Confirmed by HPLC (≥99.0%), NMR (1H, 13C), HRMS, and elemental analysis, compliant with USP standards, suitable for Cyclobenzaprine impurity analysis and quality control.

• Stability Assurance：Stable for 36 months at -20℃ under light-protected, sealed storage; degradation rate <0.3% in methanol-water mixture within 6 months.

Applications

• USP Compliance Testing：Used for UPLC-MS/MS detection of USP Related Compound A in Cyclobenzaprine API and formulations, controlling content to meet USP pharmacopoeia standards (single impurity limit ≤0.1%).

• Process Optimization Research：Monitors impurity formation during Cyclobenzaprine synthesis, reducing generation by >40% by adjusting cyclization temperature (e.g., 80-90℃) and reaction time.

• Method Validation：Serves as a USP reference standard for developing impurity detection methods, verifying UPLC resolution (≥3.0) and LOD (0.01 ng/mL).

Background Description

Research Status

• Detection Technology：UPLC-MS/MS with C18 column (1.7μm) and 0.1% formic acid-acetonitrile gradient elution achieves separation within 6 minutes, with LOD of 0.005 ng/mL meeting USP pharmacopoeia trace analysis requirements.

• Formation Mechanism：Formed by hydration of 5-(3-(dimethylamino)propyl)-5H-dibenzo[a,d][7]annulene under acidic conditions (e.g., acetic acid/water system); optimizing reaction pH and solvent polarity inhibits side reactions.

• Safety Evaluation：In vitro cytotoxicity shows IC?? of 195.3 μM against C2C12 muscle cells (Cyclobenzaprine IC??=12.6 μM), with lower toxicity than the main drug but requiring strict content control. Long-term stability testing is ongoing to monitor degradation under different humidity, light, and temperature conditions.

WhatsAPP: +86 17320513646
E-mail: anna@molcoo.com

NOTE！

We can also customize related analogues and modified peptides including HPLC, MS, 1H-NMR, MS, HPLC, IR, UV, COA, MSDS.
This product is intended for laboratory use only!

WhatsAPP: +86 17320513646
E-mail: anna@molcoo.com

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