• Product Number: C125004

• English Name: Chenodeoxycholic Acid Impurity 4

• English Alias: (R)-methyl 4-((3R,5S,7R,8R,9S,10S,13R,14S,17R)-7-acetoxy-3-hydroxy-10,13-dimethylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)pentanoate

• CAS Number: 93701-16-7

• Molecular Formula: C27H44O5

• Molecular Weight: 448.64

• Impurity Detection: Used to establish and optimize the detection methods for Impurity 4 in chenodeoxycholic acid bulk drugs and formulations, such as high-performance liquid chromatography (HPLC) and liquid chromatography - mass spectrometry (LC - MS). Determine the limit of detection (LOD) and limit of quantification (LOQ), and control the impurity content to meet international standards such as ICH Q3A.

• Process Optimization: During the production of chenodeoxycholic acid, monitor the content of Impurity 4 in real time. Analyze the impact of factors such as reaction temperature, time, raw material ratio, and catalyst use on its generation, optimize the synthesis process, reduce impurity generation, and improve product purity and quality.

• Stability Studies: In drug stability tests (accelerated tests, long-term tests), track the change trend of Impurity 4 content, analyze its impact on drug stability, and provide data support for determining the shelf life and storage conditions of drugs.

• Regulatory Compliance: Assist pharmaceutical companies in meeting the requirements of domestic and international drug regulatory agencies (such as FDA, EMA, NMPA) for drug impurity limits. Provide accurate impurity detection data during the drug registration and application process to ensure that products meet regulatory standards.

• Detection Technology: Currently, the mainstream detection method is LC - MS/MS. By optimizing the chromatographic column (such as C18 reversed-phase column with 1.8μm particle size), the mobile phase system (gradient elution of acetonitrile - water with ammonium formate buffer), and mass spectrometry parameters (electrospray ionization source, multiple reaction monitoring mode), highly sensitive detection of Impurity 4 can be achieved, with a detection limit as low as 0.01 ng/mL, significantly improving the detection accuracy and efficiency compared with traditional HPLC methods.

• Formation Mechanism: Research shows that Impurity 4 may originate from excessive acetylation reactions during the synthesis of chenodeoxycholic acid, or from incomplete conversion of residual esterified intermediates in raw materials during subsequent reactions. Through in-depth analysis of the synthesis route and simulation experiments, it has been clarified that factors such as excessively high temperature and excessive acetylation reagents are key factors promoting its generation, providing a theoretical basis for process improvement.

• Safety Evaluation: The toxicological research on Impurity 4 is gradually being carried out. Preliminary in vitro cell experiments show that high concentrations of Impurity 4 have a certain impact on the metabolic function of hepatocytes, but the specific toxic mechanism is still unclear. At present, strict limit requirements have been set for it in drug quality standards. In the future, more animal experiments and pre-clinical studies are needed to further explore its toxic characteristics and develop more scientific and reasonable impurity control strategies.

WhatsAPP: +86 17320513646
E-mail: anna@molcoo.com

NOTE！

We can also customize related analogues and modified peptides including HPLC, MS, 1H-NMR, MS, HPLC, IR, UV, COA, MSDS.
This product is intended for laboratory use only!

WhatsAPP: +86 17320513646
E-mail: anna@molcoo.com

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