BCDA NEW
| Price | $30 | $42 | $61 |
| Package | 10mg | 25mg | 50mg |
| Min. Order: | |
| Supply Ability: | 10g |
| Update Time: | 2025-06-21 |
Product Details
| Product Name: BCDA | CAS No.: 3252-36-6 |
| Purity: 97.78% | Supply Ability: 10g |
| Release date: 2025/06/21 |
Product Introduction
Bioactivity
| Name | BCDA |
| Description | BCDA (5-bromo-4-chloroindoxyl acetate) is a chromogenic substrate utilized to detect esterase activity. |
| Cell Research | Instructions I. Reagent preparation 1. Mother solution preparation: Use an appropriate solvent (such as dimethyl sulfoxide (DMSO) or an organic solvent) to dissolve BCDA into a solution of a certain concentration. The concentration usually prepared is 0.1–1 mM. 2. Working solution preparation: Adjust the concentration of the solution according to the experimental requirements, and the common concentration range is 0.2 mM to 1 mM. The solution can be freshly prepared before the experiment. II. Operation steps 1. Sample preparation: The sample can be a solution containing esterase, cell or tissue extract. For cell or tissue samples, centrifugation or other treatment may be required to remove cell debris and obtain a clean sample suitable for esterase activity determination. 2. Reaction steps: 1) Substrate addition: Add a certain volume of BCDA solution to the sample, usually at a volume ratio of 1:1 to the esterase sample. 2) Reaction conditions: Incubate the reaction system at a suitable temperature (usually 37°C) for a certain time (usually 30–60 minutes). The specific incubation time can be optimized according to the experimental requirements. 3) Esterase catalytic reaction: Under the action of esterase, BCDA will be hydrolyzed to release bromine and chlorine products, usually obvious blue or green products, which can be detected by spectrophotometer. 3. Measurement: 1) Use a spectrophotometer to measure the absorbance (OD value) at a wavelength of 620 nm or 600 nm. The color product generated by esterase catalysis is positively correlated with the esterase activity. The higher the absorbance value, the stronger the esterase activity. 2) The esterase activity in the sample can be calculated based on the standard curve. 4. Data analysis: 1) The esterase activity is calculated by comparing the absorbance of the sample with the blank control. 2) The reaction kinetics of the esterase can be obtained by measuring the absorbance at multiple different time points. Notes: 1. Solubility: BCDA has poor solubility in water, so it is usually necessary to use organic solvents such as DMSO to prepare the solution. 2. Photosensitivity: BCDA is light-sensitive, so strong light exposure should be avoided during operation and storage to prevent substrate degradation. 3. Experimental conditions: Esterase activity may be affected by factors such as pH value and temperature, so the stability and consistency of experimental conditions should be ensured. |
| Storage | keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice./Shipping at ambient temperature. |
| Solubility Information | Ethanol : 100 mg/mL (346.58 mM), Sonication is recommended. |
| Keywords | Inhibitor | inhibit | BCDA | 5-bromo-4-chloroindoxyl Acetate |
Company Profile Introduction
Target Molecule Corp. (TargetMol) is a global high-tech enterprise, headquartered in Boston, MA, specializing in chemical and biological research product and service to meet the research needs of global customers.
TargetMol has evolved into one of the biggest global compound library and small molecule suppliers and a customer based on 40+ countries. TargetMol offers over 80 types of compound libraries and a wide range of high-quality research chemicals including inhibitors, activator, natural compounds, peptides, inhibitory antibodies, and novel life-science kits, for laboratory and scientific use. Besides, virtual screening service is also available for customers who would like to conduct the computer-aided drug discovery.
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