Amine-PEG3-Biotin NEW
| Price | $30 | $43 | $58 |
| Package | 10mg | 25mg | 50mg |
| Min. Order: | |
| Supply Ability: | 10g |
| Update Time: | 2025-07-10 |
Product Details
| Product Name: Amine-PEG3-Biotin | CAS No.: 359860-27-8 |
| Purity: 98.12% | Supply Ability: 10g |
| Release date: 2025/07/10 |
Product Introduction
Bioactivity
| Name | Amine-PEG3-Biotin |
| Description | Amine-PEG3-Biotin can be used as a signal amplification label. |
| Cell Research | 1. Binding to biological molecules 1. Labeling reaction: Amine-PEG3-Biotin reacts with the target biological molecule, usually by amidation reaction to connect the dye molecule to the target molecule. Depending on the desired labeling effect, the reaction conditions, such as pH, reaction time and temperature, may need to be optimized. 2. Purification of labeled molecules: After the reaction is completed, unreacted biotin is usually removed by dialysis or chromatography. 3. Detection: Detection is performed using streptavidin-enzyme or streptavidin-fluorescent markers to amplify the signal for analysis. 2. Western Blot and immunoassay 1. Labeling antibodies: Enhance the detection sensitivity by labeling Amine-PEG3-Biotin with primary or secondary antibodies. 2. Detection: The labeled antibody is used for protein transfer and detection is performed using streptavidin-enzyme or streptavidin-fluorescent markers. 3. Signal amplification: The strong binding of biotin to streptavidin enables the signal to be amplified, improving the sensitivity of immunoassay. III. ELISA (Enzyme-Linked Immunosorbent Assay) 1. Coating: Coat the biotin-labeled target molecule onto the ELISA plate (such as a labeled antibody or antigen). 2. Detection: Detection is performed by adding streptavidin-enzyme conjugates and generating color or signal through substrate reaction. IV. Cell experiment 1. Labeling cells: Add Amine-PEG3-Biotin to the cell culture medium and incubate for a certain period of time (usually 30 minutes to 1 hour). 2. Washing: Wash away unbound dye with PBS. 3. Fluorescence microscopy: Use a fluorescence microscope or flow cytometer for detection to observe the staining pattern and dynamic process of the cells. |
| Storage | keep away from direct sunlight | Pure form: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature. |
| Solubility Information | H2O : 95 mg/mL (226.97 mM), Sonication is recommended. DMSO : 245 mg/mL (585.35 mM), Sonication is recommended. |
| Keywords | Inhibitor | inhibit | Amine-PEG3-Biotin | Amine-PEG-3-Biotin | AminePEG3Biotin | Amine PEG3 Biotin |
Company Profile Introduction
Target Molecule Corp. (TargetMol) is a global high-tech enterprise, headquartered in Boston, MA, specializing in chemical and biological research product and service to meet the research needs of global customers.
TargetMol has evolved into one of the biggest global compound library and small molecule suppliers and a customer based on 40+ countries. TargetMol offers over 80 types of compound libraries and a wide range of high-quality research chemicals including inhibitors, activator, natural compounds, peptides, inhibitory antibodies, and novel life-science kits, for laboratory and scientific use. Besides, virtual screening service is also available for customers who would like to conduct the computer-aided drug discovery.
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United States- Since: 2011-01-07
- Address: 36 Washington Street, Wellesley Hill, MA
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