6-FAM NEW
| Price | $76 |
| Package | 100mg |
| Min. Order: | |
| Supply Ability: | 10g |
| Update Time: | 2025-07-01 |
Product Details
| Product Name: 6-FAM | CAS No.: 3301-79-9 |
| Purity: 98.73% | Supply Ability: 10g |
| Release date: 2025/07/01 |
Product Introduction
Bioactivity
| Name | 6-FAM |
| Description | 6-Carboxyfluorescein (6-FAM) contains a carboxylic acid functional group, facilitating reactions with primary amines through carbodiimide activation. |
| In vitro | 6-FAM labeled oligonucleotide probes for fluorescence in situ hybridization (FISH) I. Solution preparation: 1. Preparation of stock solution: Dissolve 6-FAM in DMSO or ethanol to prepare a 1-10 mM stock solution; (it is recommended to store at -20 ℃ or -80 ℃ in the dark after aliquoting) 2. Preparation of working solution: Dilute the 6-FAM stock solution with PBS or other suitable buffer, usually 1-10 μM; (Select the appropriate working solution concentration according to experimental requirements, and prepare it for immediate use) II. Operation steps: 1. Sample preparation: Prepare chromosome samples of chicken embryonic cells, including colchicine treatment, hypotonic swelling and methanol-glacial acetic acid fixation. 2. Pretreatment: Chromosomes are treated with RNase A, pepsin and formaldehyde. 3. Hybridization: 6-FAM labeled oligonucleotides were dissolved as probes in hybridization buffer (50% formamide, 2×SSC, 10% dextran sulfate) containing E. coli tRNA at a concentration of 20 ng/μL. After co-denaturation of DNA and probe, hybridization was performed overnight at room temperature in a humidified box; 4. Washing and counterstaining: After hybridization, the slides were washed three times in 2×SSC, dehydrated in a series of 70%-96% ethanol, and sealed with an antifade containing DAP I after drying; 5. Observation and analysis: The samples were observed using a Leica DM4000B fluorescence microscope equipped with a monochrome CCD camera DFC350FX and appropriate filters, and the chromosome color images were captured and processed using QFISH software. |
| Storage | keep away from direct sunlight,keep away from moisture | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice./Shipping at ambient temperature. |
| Solubility Information | DMSO : 35 mg/mL (93.01 mM), Sonication is recommended. |
| Keywords | Inhibitor | inhibit | 6-FAM | 6FAM | 6 FAM |
| Related Compound Libraries | Human Metabolite Library |
Company Profile Introduction
Target Molecule Corp. (TargetMol) is a global high-tech enterprise, headquartered in Boston, MA, specializing in chemical and biological research product and service to meet the research needs of global customers.
TargetMol has evolved into one of the biggest global compound library and small molecule suppliers and a customer based on 40+ countries. TargetMol offers over 80 types of compound libraries and a wide range of high-quality research chemicals including inhibitors, activator, natural compounds, peptides, inhibitory antibodies, and novel life-science kits, for laboratory and scientific use. Besides, virtual screening service is also available for customers who would like to conduct the computer-aided drug discovery.
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United States- Since: 2011-01-07
- Address: 36 Washington Street, Wellesley Hill, MA
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