5-IAF NEW
| Price | $31 |
| Package | 1mg |
| Min. Order: | |
| Supply Ability: | 10g |
| Update Time: | 2025-07-23 |
Product Details
| Product Name: 5-IAF | CAS No.: 63368-54-7 |
| Supply Ability: 10g | Release date: 2025/07/23 |
Product Introduction
Bioactivity
| Name | 5-IAF |
| Description | 5-IAF is a fluorescein derivative of indoleacetamide, a fluorescent probe that can be used to label proteins and others containing free thiols (cysteine side chains). 5-IAF can label the catalytic (alpha) subunit of dog kidney Na,K-ATPase. |
| In vitro | Instructions 1. Preparation and storage a. Solubility: 5-IAF is usually provided in powder form and is easily soluble in DMSO, DMF or aqueous solution at pH ≥ 8. Recommended stock solution concentration: 10–50 mM (adjust according to experimental needs). Before use, dissolve it in anhydrous solvent and aliquot into small volumes to avoid repeated freezing and thawing. b. Storage conditions: Powder: Store in a dry environment, away from light at -20°C or lower. Solution: Short-term storage at 4°C, long-term storage at -20°C away from light is recommended. 2. Thiol labeling a. Protein reduction treatment (if necessary): To ensure that the thiol groups are in a reduced state, protein samples can be pretreated with DTT or TCEP. Use a thiol-free buffer (such as PBS or HEPES), pH 7–8 is the optimal range. b. Labeling reaction: Dilute the stock solution of 5-IAF to an appropriate working concentration (usually 0.1–1 mM). Add to the protein solution (usually 1–10 mg/mL) and mix gently. c. Incubate at room temperature for 30 minutes to 2 hours, the specific time needs to be optimized according to the experiment. d. Termination reaction: Terminate the labeling reaction by adding excess reducing agent (such as β-mercaptoethanol) or dilution. e. Purification: Remove unbound 5-IAF and reaction byproducts using dialysis, gel filtration, or centrifugal ultrafiltration. 3. Fluorescence detection Excitation wavelength: ~495 nm. Emission wavelength: ~520 nm (green fluorescence). The fluorescent signal of the labeled protein can be detected in a fluorescence microscope or fluorescence spectrometer. |
| Storage | store at low temperature,keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature. |
| Solubility Information | DMF : 90.0 mg/mL (174.7 mM ), Sonication and heating to 60℃ are recommended. DMSO : 45.0 mg/mL (87.3 mM), Sonication is recommended. |
| Keywords | 5-IAF |
Company Profile Introduction
Target Molecule Corp. (TargetMol) is a global high-tech enterprise, headquartered in Boston, MA, specializing in chemical and biological research product and service to meet the research needs of global customers.
TargetMol has evolved into one of the biggest global compound library and small molecule suppliers and a customer based on 40+ countries. TargetMol offers over 80 types of compound libraries and a wide range of high-quality research chemicals including inhibitors, activator, natural compounds, peptides, inhibitory antibodies, and novel life-science kits, for laboratory and scientific use. Besides, virtual screening service is also available for customers who would like to conduct the computer-aided drug discovery.
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United States- Since: 2011-01-07
- Address: 36 Washington Street, Wellesley Hill, MA
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