2-(4-Hydroxyphenylazo)benzoicacid NEW
| Price | $50 |
| Package | 1g |
| Min. Order: | |
| Supply Ability: | 10g |
| Update Time: | 2025-07-30 |
Product Details
| Product Name: 2-(4-Hydroxyphenylazo)benzoicacid | CAS No.: 1634-82-8 |
| Purity: 98.71% | Supply Ability: 10g |
| Release date: 2025/07/30 |
Product Introduction
Bioactivity
| Name | 2-(4-Hydroxyphenylazo)benzoicacid |
| Description | 2-(4-Hydroxyphenylazo)benzoicacid is a spectrophotometric probe that shows changes in absorption spectra upon binding to proteins and binds to bovine serum proteins. |
| In vitro | Instructions 1. Storage solution preparation: When preparing the stock solution, deionized water or an appropriate amount of ethanol can be used, and the concentration range is usually 1–10 mM. 2. Operation steps: Binding experiment: 1. Binding of protein and probe: 1) Dissolve the target protein (such as bovine serum albumin, BSA) in an appropriate buffer (such as PBS buffer at pH 7.4). 2) Add 2-(4-Hydroxyphenylazo)benzoic Acid in a certain proportion to ensure that its final concentration is suitable for absorption spectrum measurement (such as 10–50 μM). 2. Incubation conditions: Incubate the mixed system at room temperature or 37°C for 10–30 minutes to ensure that the probe is fully bound to the protein. 3. Absorption spectrum detection: Use a spectrophotometer to scan the absorption spectrum changes in the range of 350–500 nm. After the probe binds to the protein, its characteristic absorption peak (usually around 400 nm) will change significantly. 4. Data analysis: Compare the absorption spectra of the free probe after binding to the protein to determine the binding kinetic parameters. Use titration experiments (such as gradually increasing the probe or protein concentration) to draw binding curves and calculate the binding constant. Notes: 1) The probe is sensitive to pH and a suitable buffer should be used to stabilize the experimental conditions. 2) Prevent light from causing compound degradation or protein denaturation. 3) Before the measurement, ensure that the solution is free of bubbles and particles to avoid interfering with spectral detection. |
| Storage | keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature. |
| Solubility Information | Ethanol : 18 mg/mL (74.31 mM), Sonication is recommended. |
| Keywords | Dye reagent |
Company Profile Introduction
Target Molecule Corp. (TargetMol) is a global high-tech enterprise, headquartered in Boston, MA, specializing in chemical and biological research product and service to meet the research needs of global customers.
TargetMol has evolved into one of the biggest global compound library and small molecule suppliers and a customer based on 40+ countries. TargetMol offers over 80 types of compound libraries and a wide range of high-quality research chemicals including inhibitors, activator, natural compounds, peptides, inhibitory antibodies, and novel life-science kits, for laboratory and scientific use. Besides, virtual screening service is also available for customers who would like to conduct the computer-aided drug discovery.
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United States- Since: 2011-01-07
- Address: 36 Washington Street, Wellesley Hill, MA
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