1-Methyl-7-nitroisatoic anhydride NEW
| Price | $59 | $76 |
| Package | 25mg | 50mg |
| Min. Order: | |
| Supply Ability: | 10g |
| Update Time: | 2025-07-24 |
Product Details
| Product Name: 1-Methyl-7-nitroisatoic anhydride | CAS No.: 73043-80-8 |
| Purity: 98.9% | Supply Ability: 10g |
| Release date: 2025/07/24 |
Product Introduction
Bioactivity
| Name | 1-Methyl-7-nitroisatoic anhydride |
| Description | 1-Methyl-7-nitroisatoic anhydride (1M7) is a reagent. It detects local nucleotide flexibility, for probing 2'-hydroxyl reactivity. |
| In vitro | 1-Methyl-7-nitroisatoic anhydride RNA shape and structure processing experiment a. Preparation of solutions and samples: 1. Solution preparation: 1.1 Mother solution preparation: Prepare a certain concentration of 1M7 mother solution, store it at -20℃ or -80℃ in the dark after aliquoting. 1.2 Working solution preparation: Select the appropriate working solution concentration according to the experimental requirements, such as diluting it into a 1M7 working solution with a concentration of 80 mM, and try to prepare it before use. 2. Preparation of sample RNA: RNA should be dissolved in RNase-free water or 0.5× TE buffer pH 8.0 at a concentration of 5 μM, and aliquot and store at ?20 °C. b. Operation steps: 1. 1 μL 80 mM 1M7 + 9 μL RNA, mix quickly by pipetting and incubate at 37 °C for 3 minutes. The incubation time of the 1M7 reagent depends on the temperature and pH of the system being studied. Incubation time will be longer at lower temperatures and more acidic pH values. 2. Ethanol precipitation. Add 90 μL RNase-free water, 4 μL 5 M NaCl, 1 μL glycogen (20 mg/mL), and 240 μL ethanol to the mixture. Stir well. Incubate at ?80°C for 30 minutes. 3. Centrifuge at maximum speed in a microcentrifuge at 4°C for 30 minutes before subsequent primer extension experiments. |
| Storage | keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature. |
| Solubility Information | DMSO : 50 mg/mL (225.07 mM), Sonication is recommended. |
| Keywords | Inhibitor | inhibit | 1-Methyl-7-nitroisatoic anhydride | 1Methyl7nitroisatoic anhydride | 1 Methyl 7 nitroisatoic anhydride |
Company Profile Introduction
Target Molecule Corp. (TargetMol) is a global high-tech enterprise, headquartered in Boston, MA, specializing in chemical and biological research product and service to meet the research needs of global customers.
TargetMol has evolved into one of the biggest global compound library and small molecule suppliers and a customer based on 40+ countries. TargetMol offers over 80 types of compound libraries and a wide range of high-quality research chemicals including inhibitors, activator, natural compounds, peptides, inhibitory antibodies, and novel life-science kits, for laboratory and scientific use. Besides, virtual screening service is also available for customers who would like to conduct the computer-aided drug discovery.
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United States- Since: 2011-01-07
- Address: 36 Washington Street, Wellesley Hill, MA
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