97207-47-1
97207-47-1 結(jié)構(gòu)式
基本信息
甲異靛
甲異靛, >96%
1-甲基-[3,3'-聯(lián)吲哚基] -2,2'-二酮
N-甲基-(3,3'-雙-二氫吲哚)-2,2'-二酮
Natura-α
Dian III
MEISOINDIGO
Meisoindigo, >96%
Methylisoindigotin
Meisoindigo, >=98%
Meisoindigo(Natura-
N-methylisoindigotin
Meisoindigo USP/EP/BP
物理化學(xué)性質(zhì)
常見問題列表
59-48-3
2058-74-4
97207-47-1
通用方法：將1-甲基靛紅（1, 1.0 mmol）、2-吲哚酮（2, 1.0 mmol）和四氯化鋯（ZrCl4, 23 mg, 0.1 mmol）的混合物在無水乙醇（5 mL）中于回流條件下加熱。反應(yīng)進(jìn)程通過薄層色譜（TLC）監(jiān)測，待反應(yīng)物完全消失（通常需要8-12小時(shí)）后，將反應(yīng)混合物緩慢冷卻至室溫。析出的紅色固體通過過濾收集，并用少量無水乙醇洗滌，得到純的1-甲基-[3,3'-聯(lián)吲哚啉亞基]-2,2'-二酮（3）。
參考文獻(xiàn)：
[1] Tetrahedron Letters, 2016, vol. 57, # 52, p. 5856 - 5858
[2] Bulletin de la Societe Chimique de France, 1914, vol. <4> 15, p. 335
[3] Comptes Rendus Hebdomadaires des Seances de l'Academie des Sciences, 1913, vol. 156, p. 1385
Meisoindigo (Dian III; 5-20 μM; for 24?hours) inhibits growth of the AML cell lines.
Meisoindigo (10?μM; for 24?hours) induces apoptosis of acute myeloid leukemia.
Meisoindigo (5-10?μM; for 24?hours) causes cell-cycle arrest.
Meisoindigo (5-10?μM; for 24?hours) increases the cleaved caspase-3 and pro-apoptotic Bak, and decreases Bcl-2 and Bcl-xL levels in HL-60 cells.
Meisoindigo (10, 30, 50, 100, 150 μM; 24 hours) interdicts LPS-induced (1 μg/mL) NLRP3 inflammasome activation and M1/M2 polarization through down-regulation of TLR4 pathways after OGD/R in HT-22 and BV2 cells.
Cell Viability Assay
| Cell Line: | HL-60, NB4, U937 leukemic cell lines |
| Concentration: | 5, 10, 15, 20 μM |
| Incubation Time: | For 24?hours |
| Result: | Inhibited growth of the AML cell lines in a dose- and time-dependent manner. |
Apoptosis Analysis
| Cell Line: | HL-60 cells |
| Concentration: | 10?μM |
| Incubation Time: | For 24?hours |
| Result: | Induced apoptosis of acute myeloid leukemia. |
Cell Cycle Analysis
| Cell Line: | HL-60 cells |
| Concentration: | 5, 10?μM |
| Incubation Time: | For 24?hours |
| Result: | Caused cell-cycle arrest, with more cells in sub-G1 and G0/G1 phases and fewer cells in the S phase, in a dose-dependent manner. |
Western Blot Analysis
| Cell Line: | HL-60 cells |
| Concentration: | 5, 10?μM |
| Incubation Time: | For 24?hours |
| Result: | Increased the cleaved caspase-3 and pro-apoptotic Bak, and decreased Bcl-2 and Bcl-xL levels in HL-60 cells. |
Meisoindigo (Dian III; 50-150?mg/kg; IP; daily; for 14 days) has anti-leukemic activity in?vivo.
Meisoindigo (2, 4, 8, 12 mg/kg; IP; before MCAO and 2 h after reperfusion) significantly reduces infarct volume, ameliorates neurological deficits 3 days after middle cerebral artery occlusion (MCAO) in Wild-type C57BL/6J mice (25-30 g). Meisoindigo reduces edema and lowers AQP4 expression in the brain.
| Animal Model: | NOD/SCID mice, 6-8 weeks old, with HL-60 leukemic cells |
| Dosage: | 50, 100, 150?mg/kg |
| Administration: | IP; daily; for 14 days |
| Result: | Had anti-leukemic activity in?vivo. |