210292-65-2
210292-65-2 結(jié)構(gòu)式
基本信息
Thiol-green 1
1H-Benz[de]isoquinoline-1,3(2H)-dione, 2-butyl-6-[[2-(2,5-dihydro-2,5-dioxo-1H-pyrrol-1-yl)ethyl]amino]-
物理化學(xué)性質(zhì)
| 報(bào)價(jià)日期 | 產(chǎn)品編號(hào) | 產(chǎn)品名稱 | CAS號(hào) | 包裝 | 價(jià)格 |
| 2025/05/22 | HY-D1261 | Naph-EA-mal | 210292-65-2 | 1 mg | 1363元 |
| 2025/05/22 | HY-D1261 | NAPH-EA-MAL Naph-EA-mal | 210292-65-2 | 5 mg | 3000元 |
| 2025/05/22 | HY-D1261 | Naph-EA-mal | 210292-65-2 | 10 mM * 1 mLin DMSO | 3300元 |
常見(jiàn)問(wèn)題列表
Imaging thiols in living cellsHep G2 cells incubated with Naph-EA-mal (Thiol-green 1) (1 μM;5 mins), and strong green fluorescence can be observed. When cells are preincubated the cells to block the intracellular thiols with NEM (a thiol block reagent; 100 μM), a weaker fluorescence is observed in cells.Monitoring thiol-disulfide levels in fixed cellsDue to the majority of cellular thiols are present in the reduced form, Naph-EA-mal (Thiol-green 1) (10 μM) induces strong fluorescence in Hep G2 cells. After diamide treatment, fluorescent signal sharp dimed is observed.The Hep G2 cells, oxidized by diamide (200 μM; 30 min), are fixed by 70% EtOH and the remaining free thiols are blocked by NEM (100 μM). then The cells are further treated with TCEP (5 mM) followed by addition of the Naph-EA-mal (Thiol-green 1) (10 μM). The green fluorescent signal is detected again