114458-99-0
中文名稱
四甲基羅丹明-4-碘乙酰胺
CAS
114458-99-0
分子式
C26H24IN3O4
分子量
569.391
MOL 文件
114458-99-0.mol
更新日期
2025/07/22 16:39:47
114458-99-0 結(jié)構(gòu)式
基本信息
中文別名
四甲基羅丹明-4-碘乙酰胺 英文別名
VINNHWDBTRUVKY-UHFFFAOYSA-NTetramethylrhodamine-4-iodoacetamide
Tetramethylrhodamine-5-iodoacetamide dihydroiodide
5-TMRIA [TetraMethylrhodaMine-5-IodoacetaMide Dihydroiodide]
9-[2-Carboxy-4-[(2-iodoacetyl)amino]phenyl]-3,6-bis(dimethylamino)xanthylium inner salt
常見問題列表
生物活性
Tetramethylrhodamine-5-iodoacetamide (5-TMRIA) 是一種選擇性巰基反應(yīng)染料，用于通過半胱氨酸殘基對蛋白質(zhì)進(jìn)行非特異性標(biāo)記。Tetramethylrhodamine-5-iodoacetamide (5-TMRIA) 可用于共價標(biāo)記 DNA 片段。體外研究
Tetramethylrhodamine-5-iodoacetamide (0.5, 1.0, 2.0, 3.0, 4.0, 5.0, and 7.5 mM dye) shows different results for labeling carbonic anhydrase and myoglobin. At lower dye concentrations (up to 40:1 dye:thiol ratio) fluorescence labeling of only the carbonic anhydrase band is observed. However,as concentration increases, fluorescence labeling of both carbonic anhydrase and the lower myoglobin band is observed, indicating nonspecific labeling at these dye:thiol ratios.