| Identification | Back Directory | [Name]
6-TET phosphoramidite [5'-Tetrachlorofluorescein phosphoramidite] | [CAS]
877049-90-6 | [Synonyms]
5'-Tetrachlorofluorescein CEP
6-TET phosphoramidite [5'-Tetrachlorofluorescein phosphoramidite]
6-TET phosphoramidite [5'-Tetrachlorofluorescein phosphoramidite] *CAS#: 877049-90-6*
6-(4,7,2', 7'-Tetrachloro-3', 6'-dipivaloylfluoresceinyl-6-carboxamido)-hexyl-1-O-[(2-cyanoethyI)-(N, N-diisopropyD)]-phosphoramidite | [Molecular Formula]
C46H54Cl4N3O10P | [MDL Number]
MFCD01940926 | [MOL File]
877049-90-6.mol | [Molecular Weight]
981.72 |
| Chemical Properties | Back Directory | [Boiling point ]
895.1±65.0 °C(Predicted) | [solubility ]
Good solubility in acetonitrile and DCM | [pka]
12.78±0.20(Predicted) | [Appearance]
white solid foam |
| Hazard Information | Back Directory | [Description]
TET phosphoramidite for synthesis of fluorescently labeled oligonucleotides, pure 6-isomer.
TET (tetrachlorofluorescein) is a green-fluorescent fluorescein derivate (absorption maximum at 519 nm, emission maximum at 535 nm).
TET phosphoramidite is used for synthesis of fluorescently-labeled primers and hybridization probes for qPCR. TET can be used with DusQ1 fluorescence quencher (can be used with 500 ? DusQ1 CPG 500).
5’-labeled primers are used with non-labeled reverse primers for microsatellite amplification via PCR followed by fragment analysis. TET-labeled amplification products can be analyzed using various sequencers for capillary electrophoresis, including ABI PRISM? 310 Genetic Analyzer.
Condensation: 3 min.
Deprotection: standard conditions with 25% ammonium hydroxide; deprotection time depends on oligonucleotide composition and nucleobase protecting groups (deprotection for 17 hours at 55°C removes all protecting groups from standard nucleobases). AMA (solution of 30% ammonium hydroxide/40% aqueous methylamine 1:1 v/v) can be used with ~5% non-fluorescent side product forming. To avoid formation of the side product, start deprotection with ammonium hydroxide (30 min at room temperature), then add an equal volume of 40% aqueous methylamine and continue deprotection as required with AMA (10 min at 65°C). | [Uses]
6-TET phosphoramidite (5′-Tetrachlorofluorescein phosphoramidite) is a fluorescent dye that can be used for labeling an oligonucleotide with fluorescein[1]. | [References]
[1] El-Hajj HH, et al. Detection of rifampin resistance in Mycobacterium tuberculosis in a single tube with molecular beacons. J Clin Microbiol. 2001 Nov;39(11):4131-7. DOI:10.1128/JCM.39.11.4131-4137.2001 | [Abs/Em Maxima]
519/535 nm | [Fluoresene quantum yield]
0.47 | [Extinction Coefficient]
100000 | [CF260]
0.17 | [CF280]
0.09 |
|
|