| Identification | Back Directory | [Name]
BZ-NLE-LYS-ARG-ARG-AMC | [CAS]
863975-32-0 | [Synonyms]
Bz-nLKRR-AMC
Bz-Nle-KRR-AMC
BZ-NLE-LYS-ARG-ARG-AMC
Bz-Nle-KRR-AMC (hydrochloride)
Bz-Nle-Lys-Arg-Arg-AMC trifluoroacetate salt
BZ-NLKRR-AMC;BZ-NLE-LYS-ARG-ARG-AMC;863975-32-0
L-Argininamide, N-benzoyl-L-norleucyl-L-lysyl-L-arginyl-N-(4-methyl-2-oxo-2H-1-benzopyran-7-yl)- | [Molecular Formula]
C41H60N12O7 | [MDL Number]
MFCD18782523 | [MOL File]
863975-32-0.mol | [Molecular Weight]
832.99 |
| Chemical Properties | Back Directory | [storage temp. ]
Store at -20°C | [solubility ]
DMF: 30 mg/ml; DMSO: 30 mg/ml; DMSO:PBS (pH 7.2) (1:1): 0.5 mg/ml; Ethanol: 20 mg/ml | [form ]
A solid | [Sequence]
{Bz-Nle}-Lys-Arg-Arg-AMC |
| Hazard Information | Back Directory | [Description]
Bz-Nle-KRR-AMC is a fluorogenic peptide substrate for yellow fever virus (YFV) non-structural 3 (NS3) and dengue virus (DV) NS2B/3 serine proteases. Upon enzymatic cleavage by YFV NS3 or DV NS2B/3 serine proteases, 7-amino-4-methylcoumarin (AMC) is released and its fluorescence can be used to quantify YFV NS3 or DV NS2B/3 serine protease activity. AMC displays excitation/emission maxima of 340-360/440-460 nm, respectively. | [Uses]
Bz-Nle-Lys-Arg-Arg-AMC can be used for protease activity assay. In 0.1mL Tris-HCl buffer (50 mM, pH 7.8), Bz-Nle-Lys-Arg-Arg-AMC was hydrolyzed, and the release rate of AMC was observed and calculated at λex/λem=380 nm/460 nm[1]. | [References]
[1] Phoo WW, et al. Crystal structures of full length DENV4 NS2B-NS3 reveal the dynamic interaction between NS2B and NS3. Antiviral Res. 2020 Oct;182:104900. DOI:10.1016/j.antiviral.2020.104900 |
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