| Identification | Back Directory | [Name]
QSY 21 CARBOXYLIC ACID,
SUCCINIMIDYL ESTER | [CAS]
304014-13-9 | [Synonyms]
Qsy
21
QSY21 SE
Qsy
21nhs
QSY21 NHS ester
QSY 21 CARBOXYLIC ACID,
SUCCINIMIDYL ESTER
Xanthylium, 3,6-bis(2,3-dihydro-1h-indol-
1-yl)-9-[2-[[4-[[(2,5-dioxo-1-pyrrolidinyl)oxy]carbonyl]-
1-piperidinyl]sulfonyl]phenyl]-, Chloride | [Molecular Formula]
C45H39ClN4O7S | [MOL File]
304014-13-9.mol | [Molecular Weight]
815.34 |
| Chemical Properties | Back Directory | [Melting point ]
>200℃ | [solubility ]
Soluble in dimethyl sulfoxide, methanol | [form ]
Solid | [color ]
Purple solid | [λmax]
661 nm | [Biological Applications]
Amplification assays; hybridization assays; litigation assays; ligand assays; detecting analytes,enzymes,optical microcavities,protein– DNA interactions; imaging apoptosis,b-lactamase activity; FRET probes |
| Hazard Information | Back Directory | [Uses]
QSY 21 NHS, a dark quencher is an efficient energy transfer acceptor of the far red and NIR fluorescent probes. QSY 21 NHS works in
the wavelength range of 540-750 nm, and is frequently used in FRET applications. QSY 21 NHS does not emit fluorescence in normal conditions. NHS esters can be used to label the primary amines (R-NH2) of proteins, amine-modified oligonucleotides, and other amine-containing molecules[1]. | [References]
[1] Kabelác M, et al. A comparative study of the binding of QSY 21 and Rhodamine 6G fluorescence probes to DNA: structure and dynamics. Phys Chem Chem Phys. 2010 Sep 7;12(33):9677-84. DOI:10.1039/c004020g |
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