| Identification | Back Directory | [Name]
TERT-BUTYL 12-AMINO-4 7 10-TRIOXA-DODECA | [CAS]
252881-74-6 | [Synonyms]
H2N-PEG3-tBu
NH2-PEG3-C2-Boc
Amino-PEG3-COOtBu
H2N-PEG3-CH2CH2COOtBu
NH2-PEG3-CH2CH2COOtBu
Amino-PEG3-tert-butyl ester
[(+)-BIOTIN-PEG12-NHS ESTER]
H2N-PEG3-CH2CH2-t-butylester
tert-Butyl 12-aMino-4,7,10-trioxa-dodec
TERT-BUTYL 12-AMINO-4 7 10-TRIOXA-DODECA
tert-Butyl 3-(2-(2-(2-aminoethoxy)ethoxy)ethoxy)propanoate
tert-butyl 3-(2-(2-(2-aminoethoxyl)ethoxy)ethoxy)propanoate
tert-butyl 3-{2-[2-(2-hydroxyethoxy)ethoxy]ethoxy}propanoate
tert-Butyl 12-amino-4,7,10-trioxadodecanoate technical, >=80% (T)
3-[2-[2-(2-Aminoethoxy)ethoxy]ethoxy]propionic acid tert-butyl ester
Propanoic acid,3-[2-[2-(2-aMinoethoxy)ethoxy]ethoxy]-, 1,1-diMethylethyl ester | [Molecular Formula]
C13H27NO5 | [MDL Number]
MFCD06201017 | [MOL File]
252881-74-6.mol | [Molecular Weight]
277.358 |
| Chemical Properties | Back Directory | [Boiling point ]
358.5±27.0 °C(Predicted) | [density ]
1.029±0.06 g/cm3(Predicted) | [Fp ]
169.7 °C | [storage temp. ]
−20°C
| [form ]
oil | [pka]
8.74±0.10(Predicted) | [color ]
Clear | [InChI]
InChI=1S/C13H27NO5/c1-13(2,3)19-12(15)4-6-16-8-10-18-11-9-17-7-5-14/h4-11,14H2,1-3H3 | [InChIKey]
CWFSAZJIJBTKRC-UHFFFAOYSA-N | [SMILES]
C(OC(C)(C)C)(=O)CCOCCOCCOCCN |
| Hazard Information | Back Directory | [Description]
Amino-PEG3-t-butyl ester is a PEG linker containing an amino group with a t-butyl protected carboxyl group. The hydrophilic PEG spacer increases solubility in aqueous media. The amino group is reactive with carboxylic acids, activated NHS esters, carbonyls (ketone, aldehyde) etc. The t-butyl protected carboxyl group can be deprotected under acidic conditions. | [Uses]
tert-Butyl 12-amino-4,7,10-trioxadodecanoate is generally used as a spacer or linker in bioconjugate chemistry. Some of its applications are:
- Synthesis of the tetanus-toxin conjugate of MUC1 glycopeptide antigen, as a potential antitumor vaccine.
- Synthesis of self-assembled monolayer-based surface-enhanced raman scattering (SERS) labels for immuno-SERS microscopy.
- Synthesis of polycationic adamantane-based dendrons for cell imaging.
- Synthesis of phthalocyanine (Pc)-peptide conjugates as potential fluorescence imaging agents for cancers overexpressing epidermal growth factor receptors (EGFR).
| [reaction suitability]
reagent type: cross-linking reagent | [Synthesis]
The general procedure for the synthesis of tert-butyl 12-amino-4,7,10-trioxa-dodecanoate from tert-butyl 3-(2-(2-(2-azidoethoxy)ethoxy)ethoxy)propionate was as follows: Raney-Ni (7.5 g, suspended in water) was washed sequentially with water (three times) and isopropanol (three times), and was subsequently mixed with tert-butyl 3-(2-(2-(2- azidoethoxy)ethoxy ) ethoxy)propionic acid tert-butyl ester (5.0 g, 16.5 mmol) in isopropanol. The reaction mixture was stirred at room temperature under hydrogen atmosphere for 16 hours. After completion of the reaction, the reaction mixture was filtered through a diatomaceous earth pad and the pad was washed with isopropanol. The filtrate was concentrated and purified by column chromatography (eluent: 5-25% methanol/dichloromethane) to afford the light yellow oily product tert-butyl 12-amino-4,7,10-trioxa-dodecanoate (2.60 g, 57% yield). Mass spectrometry (ESI) m/z calculated value C13H28NO5 [M+H]+ 279.19; measured value: 279.19. | [IC 50]
PEGs | [References]
[1] Organic and Biomolecular Chemistry, 2015, vol. 13, # 1, p. 81 - 97
[2] Journal of the American Chemical Society, 2018, vol. 140, # 38, p. 12189 - 12199
[3] Patent: WO2016/59622, 2016, A2. Location in patent: Page/Page column 102-103
[4] Patent: WO2017/46658, 2017, A1. Location in patent: Page/Page column 148
[5] Patent: WO2018/86139, 2018, A1. Location in patent: Page/Page column 151 |
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