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Thanatin is an inducible cationic antimicrobial peptide. Thanatin is a pathogen-inducible single-disulfide-bond-containing β-hairpin AMP. Thanatin displays broad-spectrum activity against both Gram-negative and Gram-positive bacteria as well as against various species of fungi with MICs of 0.3-40 μM, 0.6-40 μM and 0.6-20 μM, respectively. Thanatin has the property of competitive replacement of divalent cations from bacterial outer membrane (OM), leading to OM disruption[1][2]. | [in vivo]
Thanatin (1, 3, 6?mg/kg; ip; at 1 and 6?h) protects mice infected with NDM-1-producing E. coli[2].
| Animal Model: | Male BALB/c mice aged 8-10 weeks and weighing 18-22?g with CFU E. coli XJ141026 | | Dosage: | 1, 3, 6?mg/kg | | Administration: | Intraperitoneally injected; at 1 and 6?h | | Result: | 1, 3, and 6?mg/kg markedly increased the survival rate from 0 to 30%, 70%, and 100%, respectively.
Showed that the bacterial titers decreased with increasing drug dose.
Rescued the pathological damages (large amounts of inflammatory cell infiltration, alveolar fusion, congestion in the spleen red pulp area, hepatic sinusoidal dilation and congestion) in a dose-dependent manner.
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| [References]
[1] Rachita Dash, et al. Thanatin: An Emerging Host Defense Antimicrobial Peptide with Multiple Modes of Action. Int J Mol Sci. 2021 Feb 3;22(4):1522. DOI:10.3390/ijms22041522
[2] Bo Ma, et al. The antimicrobial peptide thanatin disrupts the bacterial outer membrane and inactivates the NDM-1 metallo-β-lactamase. Nat Commun. 2019 Aug 6;10(1):3517. DOI:10.1038/s41467-019-11503-3 |
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