| Identification | Back Directory | [Name]
6-Azido-L-norleucine HCl | [CAS]
1454334-76-9 | [Synonyms]
H-Lys(N3)-OH.HCl
6-Azido-L-norleucine HCl
L-Azidonorleucine hydrochloride
Nε-Azido-L-Lysine hydrochloride
(2S)-2-amino-6-azidohexanoic acid
(S)-2-Amino-6-azidohexanoic acid hydrochloride
(2S)-2-amino-6-azidohexanoic acid hydrochloride | [Molecular Formula]
C6H13ClN4O2 | [MDL Number]
MFCD18382040 | [MOL File]
1454334-76-9.mol | [Molecular Weight]
208.646 |
| Chemical Properties | Back Directory | [storage temp. ]
Store at -20°C | [solubility ]
Soluble in DMSO | [form ]
Solid | [color ]
White to off-white | [Water Solubility ]
Soluble to 100 mM in water |
| Hazard Information | Back Directory | [Uses]
L-Azidonorleucine hydrochloride, an unnatural amino acid, is A Methionine surrogate. L-Azidonorleucine hydrochloride can be used to label mammalian cell proteins and identify a diverse set of methionyl-tRNA synthetase (MetRS) mutants [1][2]. L-Azidonorleucine (hydrochloride) is a click chemistry reagent, it contains an Azide group and can undergo copper-catalyzed azide-alkyne cycloaddition reaction (CuAAc) with molecules containing Alkyne groups. It can also undergo strain-promoted alkyne-azide cycloaddition (SPAAC) reactions with molecules containing DBCO or BCN groups. | [in vivo]
When L-Azidonorleucine (ANL) is administered via the drinking water, an average daily L-Azidonorleucine intake of 0.9 mg/day/g of body weight for 21 d when aiming to label and identify neuronal populations of the brain are recommended. Lower dosages or shorter labeling times might be used for the labeling of other cell types. When L-Azidonorleucine is administered by i.p. injection, the testing different L-Azidonorleucine doses in the range of 4-400 mM are recommended. A 400 mM dosage administered by i.p. (i.p. volume: 10 ml/kg) once per day for 1 week is recommended for labeling some neuronal populations (such as excitatory neurons of the hippocampus). Lower dosages (e.g., 4 mM) can potentially be used for labeling other cell types or when longer labeling periods are desired[2]. | [storage]
Store at -20°C | [References]
[1] Alborz Mahdavi, et al. Engineered Aminoacyl-tRNA Synthetase for Cell-Selective Analysis of Mammalian Protein Synthesis. J Am Chem Soc. 2016 Apr 6;138(13):4278-81. DOI:10.1021/jacs.5b08980
[2] Beatriz Alvarez-Castelao, et al. Cell-type-specific metabolic labeling, detection and identification of nascent proteomes in vivo. Nat Protoc. 2019 Feb;14(2):556-575. DOI:10.1038/s41596-018-0106-6 |
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