| Identification | Back Directory | [Name]
Benzamide, N-[2-[[(1S)-1-(chloromethyl)-1,2-dihydro-5-hydroxy-9-methyl-3H-benz[e]indol-3-yl]carbonyl]imidazo[1,2-a]pyridin-6-yl]-4-hydroxy- | [CAS]
1227961-59-2 | [Synonyms]
Seco-DUBA
Benzamide, N-[2-[[(1S)-1-(chloromethyl)-1,2-dihydro-5-hydroxy-9-methyl-3H-benz[e]indol-3-yl]carbonyl]imidazo[1,2-a]pyridin-6-yl]-4-hydroxy- | [Molecular Formula]
C29H23ClN4O4 | [MDL Number]
MFCD34567330 | [MOL File]
1227961-59-2.mol | [Molecular Weight]
526.97 |
| Chemical Properties | Back Directory | [density ]
1.47±0.1 g/cm3(Predicted) | [form ]
Solid | [pka]
8.08±0.15(Predicted) | [color ]
Light yellow to brown | [InChIKey]
VQAFBYLFRCCWNB-GOSISDBHSA-N | [SMILES]
C(NC1=CN2C=C(C(N3C4=C(C5=C(C)C=CC=C5C(O)=C4)[C@H](CCl)C3)=O)N=C2C=C1)(=O)C1=CC=C(O)C=C1 |
| Hazard Information | Back Directory | [Uses]
Seco-DUBA is a inactive prodrug form of duocarmycin (DUBA) containing two hydroxyl groups, which can each be used for coupling to an antibody via a linker. Seco-DUBA can be used in the synthesis of antibody-drug conjugates (ADCs). | [Definition]
Duocarmycins (DUMs; A, B1, B2, C1, C2, D1, D2 and SA) are members of an extremely potent group of antitumor antibiotics isolated from Streptomyces sp. The pharmacophore consists of a cyclopropa[c]pyrrolo[3,2-e]indole (CPI) moiety. DUMs B1, B2, C1, C2, D1 and D2 were a seco-type of DUM A and chemically more stable than DUM A[1].
| [in vitro]
Seco-DUBA (SK-BR-3 cells; 0.0001 pM~0.01 nM; 144 hours) dose-dependent reduces cell viability and shows equally potent and efficacious as DUBA[1].
Seco-DUBA causes SK-BR-3 (IC50=0.09), SK-OV-3 (IC50=0.43), and SW620 (IC50=0.09) cells to exhibit highly sensitive.
Seco-DUBA (89 μg/kg; i.v.) is likely converted to DUBA almost instantaneously[2]. | [in vivo]
Seco-DUBA (89 μg/kg; i.v.) is likely converted to DUBA almost instantaneously[1]. | Animal Model: | Wistar rats[1] | | Dosage: | 89 μg/kg (Pharmacokinetic Analysis) | | Administration: | I.v. | | Result: | Likely converted to DUBA almost instantaneously.
|
| [target]
Humanized IgG1, HER-2
Humanized IgG1, 5 T4
Humanized IgG1, CD22
Mouse IgG1; CD56 | [References]
[1] Choi T, et al. Structural Influence of Indole C5-N-Substitutents on the Cytotoxicity
of seco-Duocarmycin Analogs. Archives of Pharmacal Research, 2011; 34: 357–367.
[2] Elgersma R, et al. Design, Synthesis, and Evaluation of Linker-Duocarmycin Payloads: Toward Selection of HER2-Targeting Antibody–Drug Conjugate SYD985. Molecular Pharmaceutics, 2015; 12: 1813–1835. |
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